TLC Analysis of Cranberry Juice Powder
Sample:
Dissolve 1 g of cranberry juice powder and 1 g of cranberry raw material in 10 mL of methanol, respectively for 30 minute. 
After standing for 5 minutes,the mixture is filtered. 
The filter is washed with 1 mL methanol. 
Solutions are combined and evaporated to dryness. 
The residue is dissolved in 1 mL methanol.

Chromatographic conditions:
Stationary phase: TLC plates silica gel 60 F254, 10x10 cm or 20x10 cm. 
Mobile Phase: Butyl alcohol: Acetic acid: Water
(4:1:5 V/V/V developed with the upper solution)
Sample application: 2 μL or 5 µL test solution are applied as 100 mm bands, 30 min. 10 mm apart, 100 mm from lower edge of plate.
Development: 10x10 cm or 20x10 cm Twin Trough Chamber, saturated for 20 min (filter paper), 5 mL (respectively 10 mL) developing solvent per trough developing distance 100 mm from lower edge of plate. The plate is then dried with a hair dryer (warm air) for 5 min.
Detection: Examination in natural light and 365nm.
Pls find the examination pictures as follows.
S1: Cranberry Juice Powder（2µL）
S2: Cranberry Juice Powder（5µL）
S3: Cranberry （2µL）
S4: Cranberry （5µL）

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