Method of Analysis Ginkgo biloba Extract HPLC

Content of flavonol glycosides—

Extraction solvent, Mobile phase, and Chromatographic system—Proceed as directed in the test for Content of flavonol glycosides under Ginkgo.

Standard solutions—Transfer accurately weighed quantities of USP Quercetin RS, kaempferol, and isorhamnetin to separate volumetric flasks, dissolve each in methanol, and dilute quantitatively, and stepwise if necessary, with methanol to obtain Standard solutions 1, 2, and 3 having known concentrations of 0.125 mg per mL, 0.125 mg per mL, and 0.03 mg per mL, respectively.

Test solution—Transfer about 0.300 g of Powdered Extract, accurately weighed, to a 250-mL flask fitted with a reflux condenser. Add 78 mL of Extraction solvent, and reflux on a hot water bath for 135 minutes. [note—The solution will turn deep red. The color of the solution is not a definitive indication of reaction completeness.] Allow to cool at room temperature. Transfer to a 100-mL volumetric flask, add water to volume, and mix.

Procedure—Proceed as directed in the test for Content of flavonol glycosides under Ginkgo. Calculate the percentage of each flavonol glycoside in the portion of Powdered Extract taken by the formula:

10(2.51)(C/W)(rU / rS)

in which W is the weight, in g, of Powdered Extract taken to prepare the Test solution; and the other terms are as defined therein. Calculate the total percentage of flavonol glycosides by adding the individual percentages calculated.

 

Content of terpene lactones—

Solution A, Solution B, Mobile phase, Standard solutions, Buffer solution, Diluent, and Chromatographic system—Proceed as directed in the test for Content of terpene lactones under Ginkgo.

Test solution—Transfer about 120 mg of Powdered Extract, accurately weighed, to a 25-mL beaker, and proceed as directed for Test solution in the test for Content of terpene lactones under Ginkgo, starting with “add 10 mL of Buffer solution”, except to dissolve the residue in 20.0 mL of Diluent.

Procedure—Proceed as directed in the test for Content of terpene lactones under Ginkgo. Separately calculate the percentages of bilobalide (C15H18O8), ginkgolide A (C20H24O9), ginkgolide B (C20H24O10), and ginkgolide C (C20H24O11) in the portion of Powdered Extract taken by the formula:

2000(C/W)

in which C is the concentration, in mg per mL, of the relevant analyte in the Test solution; and W is the weight, in mg, of Powdered Extract taken to prepare the Test solution. Calculate the total percentage of terpene lactones in the portion of Powdered Extract taken by adding the percentages calculated for each analyte.

Have you known the details about it?

#GinkgoBilobaExtract
#GinkgoBilobaLeafExtract #GinkgoLeafExtract #GinkgoFlavonolGlycosides #TerpeneLactones
#GinkgolicAcid
#GinkgoBilobaExtractGinkgoFlavonolGlycosides
#GinkgoBilobaLeafExtractGinkgoFlavonolGlycosides
#GinkgoLeafExtractGinkgoFlavonolGlycosides
#GinkgoBilobaExtractTerpeneLactones #GinkgoBilobaLeafExtractTerpeneLactones
#GinkgoLeafExtractTerpeneLactones
#GinkgoBilobaExtractGinkgolicAcid #GinkgoBilobaLeafExtractGinkgolicAcid
#GinkgoLeafExtractGinkgolicAcid
#Rutin #Quercetin