What is the Vitro Cytotoxicity of Aloe emodin?

The cytotoxic activity of Aloe emodin was determined in exponentially growing cells in complete medium over 72 h. The cells were seeded in 12 wells/plate 24 h before the treatment; monolayer cells were plated at a density of 5–7 × 104 cells/well, and suspension cells were plated at 40 × 104 cells/well. Aloe emodin was added to the experimental final concentration, and cells were counted 72 h later using the trypan blue exclusion assay. All of the experiments were conducted at least in triplicate.

Hemopoietic Progenitors and Neuroblastoma Colony Assay.

MNCs from BM aspirates and CB samples and from neuroblastoma cell lines (SJ-N-KP and AF8) were cultured in methylcellulose medium supplemented with a combination of recombinant colony-stimulating factors (Stem Cell Technologies, Vancouver, British Columbia, Canada). Cells were plated in triplicate at the concentration of 5 × 104/ml for BM- and CB-MNC, and 1 × 10 3 for NB cells, in 35-mm-diameter dishes (Becton Dickinson, Franklin Lakes, NJ) and incubated at 37°C in a 5% CO2 humidified atmosphere. MNC and NB cell lines were cultured in the absence or in the presence of different concentrations of Aloe emodin. On day 14 of culture, the number of CFU-GM and neuroblastoma colonies was counted with an inverted microscope (Leitz-Diavert). All of the experiments were conducted at least three times.

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